Structural and functional characterization of NEMO cleavage by SARS-CoV-2 3CLproArticle Published on 2022-09-082022-11-15 Journal: Nature Communications [Category] SARS, 진단, 치료제, [키워드] 3CLpro accounted addition binding cleavage cleave computational method COVID-19 Efficiency Essential explain functional help Host Host immune response humans Hydrogen bond hydrophobic immune in viral In vitro assay information machine modulator NEMO NF-κB pathology polyprotein predict protease Proteins residue reveal SARS-CoV-2 Signaling specificity substrate the SARS-CoV-2 Variation [DOI] 10.1038/s41467-022-32922-9 PMC 바로가기
IL4I1 binds to TMPRSS13 and competes with SARS-CoV-2 spikeArticle Published on 2022-09-052022-11-16 Journal: Frontiers in Immunology [Category] SARS, 유전자 메커니즘, [키워드] Adaptive immune response bind binding Cancer Cancer therapy cells cleavage cleave enzyme expressed expression facilitate homology identify IL4I1 immune immunosuppressive immunosuppressive enzymes Immunotherapy involved Lymphocytes mechanism of action monocyte Monocyte-derived macrophages moonlighting protein negative control Protease activity Protein Pseudotyped virus Region SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike SARS-CoV-2 spike protein secreted Spike. surface protein survival the spike protein TMPRSS13 TMPRSS2 transmembrane serine protease virus [DOI] 10.3389/fimmu.2022.982839 PMC 바로가기
Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffiiArticle Published on 2022-09-052022-11-16 Journal: Microbial Cell Factories [Category] SARS, 변종, [키워드] addition addressed aggregation Alpha Amino acid applied benefit caused Cell cleavage cleave demonstrated eliminated extension extracellular space functional Golgi highest Host IMPROVE Modification N-terminal N-terminus observation occur peptide Pichia Pichia pastoris product Product quality protease Protein protein engineering protein sequence Proteins purification Receptor binding domain Recombinant protein recombinant proteins residue Saccharomyces cerevisiae secrete secreted secreted protein secretion signal signal peptide signal peptide. steric steric hindrance subunit the SARS-CoV-2 the signal peptide therapeutic vaccine antigen variant variants [DOI] 10.1186/s12934-022-01905-2 PMC 바로가기
Exploring antiviral and anti-inflammatory effects of thiol drugs in COVID-19Article Published on 2022-09-012022-11-15 Journal: American journal of physiology. Lung cellular and [Category] COVID19(2023년), SARS, 치료제, [키워드] ACE-2 ACE2 addition airway alveolar angiotensin-converting enzyme 2 anti-inflammatory effect Anti-inflammatory effects anti-inflammatory property antioxidant Antiviral Antiviral effect antiviral effects binding cell entry cleavage cleave Concentration COVID-19 cysteamine decrease determine disrupt drug drugs Effect effective functional hamster hemorrhage in vivo infected with SARS-CoV-2 Inflammation inhibit Injury intraperitoneally limit lung Lung inflammation Lung injury mechanism neutrophilic oxidative stress pseudovirus redox status reporter SARS-2-S SARS-CoV-2 SARS-CoV-2 cell SARS-CoV-2 spike glycoprotein SARS-CoV-2 viruses significantly tested thiol drugs. thiol group viral infection virus infection [DOI] 10.1152/ajplung.00136.2022 PMC 바로가기
Flipped over U: structural basis for dsRNA cleavage by the SARS-CoV-2 endoribonuclease뒤집힌 U: SARS-CoV-2 엔도리보뉴클레아제에 의한 dsRNA 절단의 구조적 기초Article Published on 2022-08-122022-09-11 Journal: Nucleic Acids Research [Category] SARS, 유전자 메커니즘, 치료법, [키워드] activate Activation active site cleavage cleave coronavirus coronavirus Coronavirus replication Critical cryo-EM structure cryo-EM structures double-stranded dsRNA endoribonuclease evade form generate Host immune MDA5 mechanism Nsp15 Pattern recognition pattern recognition receptor platform processing recognize replication-transcription complex RNA SARS-CoV-2 SARS-CoV-2 Nsp15 single-stranded site-directed mutagenesis structures substrates the SARS-CoV-2 uridine Vesicle viral replication [DOI] 10.1093/nar/gkac589 PMC 바로가기 [Article Type] Article
Paxlovid: Mechanism of Action, Synthesis, and In Silico StudyPaxlovid: 작용 메커니즘, 종합 및 인 실리코 연구Review Published on 2022-07-072022-09-11 Journal: BioMed Research International [Category] COVID19(2023년), SARS, 치료제, [키워드] Action antiviral activity antiviral drug antiviral efficacy antivirus antiviruses Biochemistry candidate cleave clinical trial Combination coronavirus COVID COVID-19 COVID-19 pandemic described description drug flexible Glutamine Human coronavirus Immune profile in vitro in vivo inhibiting inhibitor mechanism mechanism of action molnupiravir Nirmatrelvir Organic chemistry pandemic Paxlovid PF-07321332 Pfizer Pharmacology polyprotein protease Protease inhibitor Proteases replaced REPLACED BY reported Ritonavir SARS-CoV-2 protease synthesis Treatment treatment of COVID-19 Vaccine viral replication [DOI] 10.1155/2022/7341493 PMC 바로가기 [Article Type] Review
Comprehensive fitness landscape of SARS-CoV-2 M pro reveals insights into viral resistance mechanismsSARS-CoV-2 M pro의 종합적인 피트니스 환경은 바이러스 저항 메커니즘에 대한 통찰력을 보여줍니다Article Published on 2022-06-202022-09-11 Journal: eLife [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료제, [키워드] active site acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 addition Amino acid amino acid changes amino acids analyzed Anti-viral drugs can be used cleave Complete Comprehensive conserved contribute Coronavirus-2 correlated Critical deep mutational scanning deep sequencing defined drug drug design drug resistance Effect effective elicit evade Evolution Evolutionary biology expression functional human population Infectious disease inhibitor inhibitors life cycle M pro main protease mechanism Microbiology Microbiology. MPro Mutation network new strain performed physically protease Protein reduced reveal S. cerevisiae SARS-CoV-2 SARS-CoV-2 main protease selection pressure Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus Toxicity Vaccines variant viral life cycle Viral resistance virulent yeast [DOI] 10.7554/eLife.77433 PMC 바로가기 [Article Type] Article
ORFeome Phage Display Reveals a Major Immunogenic Epitope on the S2 Subdomain of SARS-CoV-2 Spike ProteinORFeome 파지 디스플레이, SARS-CoV-2 스파이크 단백질의 S2 하위 도메인에 대한 주요 면역원성 에피토프 공개Article Published on 2022-06-172022-09-11 Journal: Viruses [Category] COVID19(2023년), MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] Amino acid amino acid sequence anti-SARS-CoV-2 antibodies antibodies antibody Antibody Response Antibody responses applied approved binding binding site blocking cell entry cleave COVID-19 COVID-19 pandemic COVID-19 patients Diseases display Efficacy epitope epitope mapping Epitopes fusion peptide genomic genomic library Host host cell identify immunogenic library Major mechanism monoclonal antibody mutate Mutation neutralization neutralize NGS Open reading frame open reading frames ORFeome ORFs Particle peptide phage phage display. RBD RBD region Receptor binding domain representing SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 variant SARS-CoV-2 variants sequence Serine serine protease serine protease TMPRSS2 spike the cell the RBD the SARS-CoV-2 genome therapeutic therapy TMPRSS2 Vaccine virus variants [DOI] 10.3390/v14061326 PMC 바로가기 [Article Type] Article
An ADAM17-Neutralizing Antibody Reduces Inflammation and Mortality While Increasing Viral Burden in a COVID-19 Mouse ModelADAM17 중화 항체는 COVID-19 마우스 모델에서 바이러스 부담을 증가시키면서 염증과 사망률을 감소시킵니다Article Published on 2022-06-102022-09-12 Journal: Frontiers in Immunology [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] ACE2 ADAM17 ADAM19 adverse outcome Alter angiotensin anti-viral role antibody appear blockade blocked burden Cell cell entry receptor cellular activation cleave COVID-19 COVID-19 pathogenesis COVID-19 patients Critical ectodomain Increasing Inflammation Inflammatory inflammatory cascade K18-hACE2 less lung Lung pathology lung; metalloproteinase mice Model monoclonal antibody mouse mouse model pathway Proinflammatory cytokine promote protease Proteins SARS-CoV SARS-CoV-2 SARS-CoV-2 viruses significantly increased significantly lower therapeutic TNF-α Transgenic mouse transmembrane viral burden virus virus. while [DOI] 10.3389/fimmu.2022.918881 PMC 바로가기 [Article Type] Article
ADAM10 and ADAM17 promote SARS-CoV-2 cell entry and spike protein-mediated lung cell fusionADAM10 및 ADAM17은 SARS-CoV-2 세포 진입 및 스파이크 단백질 매개 폐 세포 융합을 촉진합니다Article Published on 2022-06-072022-09-11 Journal: EMBO Reports [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] A549 ADAM10 ADAM17 Alpha antiviral drug apratastat Beta causative agent Cell Cell fusion cleave contribute contributing to COVID-19 COVID-19 pathogenesis COVID-19 pathology DPC-333 ectodomain ectodomain shedding expressed facilitator hallmark Host host cell host cell factor host cell factors human lung human lung cell identify impair in vitro Infection inhibitor lung cell omicron Pathogenesis pathology priming promote protease Proteases reduce required SARS-CoV-2 SARS-CoV-2 cell SARS-COV-2 infection SARS-CoV-2 spike protein SARS-CoV-2 variants Spike protein syncytia formation syncytia formation. target the SARS-CoV-2 TMPRSS2 TMPRSS2 protease variants of concern viral entry [DOI] 10.15252/embr.202154305 PMC 바로가기 [Article Type] Article